bio 21111 cell stimulation cocktail 500x ebioscience (Thermo Fisher)
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bio 21111 cell stimulation cocktail 500x ebioscience
Bio 21111 Cell Stimulation Cocktail 500x Ebioscience, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+21111+cell+stimulation+cocktail+500x+ebioscience/pm31027998-226-26-31
Average 86 stars, based on 1 article reviews
Bio 21111 Cell Stimulation Cocktail 500x Ebioscience, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+21111+cell+stimulation+cocktail+500x+ebioscience/pm31027998-226-26-31
Average 86 stars, based on 1 article reviews
bio 21111 cell stimulation cocktail 500x ebioscience - by Bioz Stars,
2026-09
86/100 stars
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Activation Assay:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Cell Stimulation:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. 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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. 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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A SYBR Green Assay:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Reporter Assay:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Enzyme-linked Immunosorbent Assay:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Chromatin Immunoprecipitation:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Multiplexing:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Sample Prep:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Gel Extraction:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Polymerase Chain Reaction:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Purification:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Selection:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Cell Isolation:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Microarray:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A RNA Sequencing Assay:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Methylated DNA Immunoprecipitation Sequencing:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A Modification:Article Title: Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PPAR-g as determinant for VAT Treg-cell gene expression Dataset Cipolletta et al., 2012; Cipolletta et al., 2015 and Li et al., 2018 GEO: GSE37535 and GSE113393 Murine Treg-cell Signature Hill et al., 2007 GEO: GSE7460 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID: CVCL_0063 Jurkat DSMZ DSMZ Cat# ACC-282, RRID: CVCL_0065 3T3-L1 ATCC Cat# CCL-92.1, RRID: CVCL_0123 Experimental Models: Organisms/Strains Foxp3YFP-Cre JAX Published in Rubtsov et al., 2008 Hpgdfl/fl Laboratory of Prof. Louis J. Muglia, Cincinnati’s Children Hospital Published in Roizen et al., 2008 Cd4Cre JAX Published in Lee et al., 2001 Ppargfl/fl JAX Published in He et al., 2003 Foxp3IRES-RFP JAX Published in Wan and Flavell, 2005 Rag2 / JAX Published in Shinkai et al., 1992 Oligonucleotides For oligonucleotide sequences, see Table S7 This paper N/A Software and Algorithms FlowJo BD Biosciences RRID: SCR_008520 LightCycler Software Roche RRID: SCR_012155 TopHat v2.0.11 http://tophat.cbcb.umd.edu/ RRID: SCR_013035 Partek Genomics Suite (PGS) Partek Inc RRID: SCR_011860 Kallisto v0.44 s https://pachterlab.github.io/ kallisto/about RRID: SCR_016582 CellProfiler Image Analysis Software 2.0 http://cellprofiler.org RRID: SCR_007358 Cytoscape http://cytoscape.org RRID: SCR_003032 BiNGO: A Biological Networks Gene Ontology tool http://www.psb.ugent.be/cbd/ papers/BiNGO/Home.html RRID: SCR_005736 Graph Pad Prism GraphPad Software RRID: SCR_002798 Fiji ImageJ http://fiji.sc RRID: SCR_002285 Other mCD1d PBS-57 BV421 NIH N/A mCD1d unloaded BV421 NIH N/A |